Biolegend, 400702, Purified Rat IgG2c, κ Isotype Ctrl Antibody, 500μg

The isotype of RTK4174 immunoglobulin is rat IgG2c, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
500μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICFC, ICC, IHC, IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: The RTK4174 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The ULEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400732-400737) as negative control.
Application References(PubMed link indicates BioLegend citation): Ganeshah K, et al. 2012. J. Immunol. 188:594. PubMed D'Agostino PM, et al. 2012. PNAS. 109:6175. PubMed
Product Citations: Maor-Nof M, et al. 2013. Cell Rep. 971:3. PubMed D'Agostino P, et al. 2012. Proc Natl Acad Sci U S A. 109:6175. PubMed Ray HC, et al. 2020. Sci Rep. 10:15808. PubMed Prideaux M, et al. 2021. Sci Rep. 11:22593. PubMed Bryce K 2012. J Immunol. 188:594. PubMed
RRID: AB_326567 (BioLegend Cat. No. 400701) AB_326568 (BioLegend Cat. No. 400702)
Gene ID: NA
Clone: RTK4174
Regulatory Status: RUO
Isotype: Rat IgG2c, κ