Biolegend, 400723, PerCP/Cyanine5.5 Rat IgG2c, κ Isotype Ctrl Antibody, 25μg

The isotype of RTK4174 immunoglobulin is rat IgG2c, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this rat IgG2c, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The RTK4174 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The ULEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400732-400737) as negative control.
Application References(PubMed link indicates BioLegend citation): Ganeshah K, et al. 2012. J. Immunol. 188:594. PubMed D'Agostino PM, et al. 2012. PNAS. 109:6175. PubMed
RRID: AB_2864281 (BioLegend Cat. No. 400723)
Gene ID: NA
Clone: RTK4174
Regulatory Status: RUO
Isotype: Rat IgG2c, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.