Biolegend, 400902, Purified Armenian Hamster IgG Isotype Ctrl Antibody, 500μg

This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
500μg
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC, ICFC - Quality tested IP, ChIP - Verified ICC, IHC, WB, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA) For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717. Yu R, et al. 2006. Obesity 14:1353. Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed
Product Citations: Martinez LE, et al. 2020. J Virol. 94:00:00. PubMed Duran CL, et al. 2023. Breast Cancer Res. 25:37. PubMed Digomann D, et al. 2023. Cancers (Basel). 15:. PubMed Gomes P, et al. 2023. Cell Commun Signal. 21:35. PubMed Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed Nie S, et al. 2019. Cancer Manag Res. 11:10257. PubMed Cannons JL, et al. 2021. Cell Rep. 37:109804. PubMed Hasezaki T, et al. 2020. Sci Rep. 10:6969. PubMed Enriquez AB, et al. 2022. iScience. 25:104305. PubMed Olcina MM et al. 2018. Cell reports. 25(13):3721-3732 . PubMed Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed Enriquez AB, et al. 2022. iScience. 25:104305. PubMed Goldstein H 2009. J Am Soc Nephrol. 1.55. PubMed Sundararaj K, et al. 2021. Immunology. 162:418. PubMed Yang J, et al. 2005. Rheumatology. 44:1245. PubMed Bechelli J, et al. 2019. Infect Immun. 87:. PubMed Taylor MD, et al. 2020. Front Immunol. 11:1946. PubMed Kalamakis G, et al. 2019. Cell. 176:1407. PubMed Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed Lagarrigue F, et al. 2018. Blood Adv. 1.720833333. PubMed Sharma VP, et al. 2021. Nat Commun. 12:7300. PubMed Sainson RCA, et al. 2020. Cancer Immunol Res. 1.422222222. PubMed Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed
RRID: AB_3096169 (BioLegend Cat. No. 400901) AB_3096169 (BioLegend Cat. No. 400902)
Gene ID: NA
Clone: HTK888
Regulatory Status: RUO
Isotype: Armenian Hamster IgG