Biolegend, 400904, Biotin Armenian Hamster IgG Isotype Ctrl Antibody, 200μg

This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
200μg
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717. Yu R, et al. 2006. Obesity 14:1353. Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed
Product Citations: Xing H, et al. 2022. Sci Rep. 12:22474. PubMed Sandow JJ, et al. 2021. Proteomics. 21:e2000244. PubMed Peraino J, et al. 2013. Hum Immunol. 18:88. PubMed Lesley R, et al. 2006. Proc Natl Acad Sci U S A. 103:10717. PubMed
RRID: AB_3094652 (BioLegend Cat. No. 400903) AB_3094652 (BioLegend Cat. No. 400904)
Gene ID: NA
Clone: HTK888
Regulatory Status: RUO
Isotype: Armenian Hamster IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.