Biolegend, 400921, PE/Cyanine7 Armenian Hamster IgG Isotype Ctrl Antibody, 25μg

This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25μg
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717. Yu R, et al. 2006. Obesity 14:1353. Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed
Product Citations: Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed Corria-Osorio J, et al. 2023. Nat Immunol. 24:869. PubMed Ocaña-Guzman R, et al. 2022. Cells. 11: . PubMed Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed Wei H, et al. 2021. Malar J. 20:89. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Zikmund T, et al. 2019. J Immunol. 202:3434. PubMed Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
RRID: AB_2905473 (BioLegend Cat. No. 400921) AB_2905473 (BioLegend Cat. No. 400922)
Gene ID: NA
Clone: HTK888
Regulatory Status: RUO
Isotype: Armenian Hamster IgG