Product Description
The HTK204 immunoglobulin is of Armenian Hamster IgM isotype and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
50μg
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC, ICFC - Quality tested WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 85% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, functional assay. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401006) as negative control.
Product Citations: Bettini S, et al. 2017. Sci Rep. 40373:7. PubMed Manteghi S, et al. 2016. Cell Rep. 15: 1893-1900. PubMed Bundesmann M, et al. 2011. Am J Respir Cell Mol Biol. 46:233. PubMed
RRID: AB_326608 (BioLegend Cat. No. 401002)
Gene ID: NA
Clone: HTK204
Regulatory Status: RUO
Isotype: Armenian Hamster IgM
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Collaboration
Tony Tang
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