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BRAND / VENDOR: Biolegend

Biolegend, 401201, Purified Mouse IgG2b, κ Isotype Ctrl Antibody, 50μg

CATALOG NUMBER: 401201
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Product Description

The isotype of MG2b-57 immunoglobulin is mouse IgG2b,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
50μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application: FC - Quality tested ChIP - Verified ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular flow cytometry, Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, functional assay. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 401215-401220) as negative control.
Application References(PubMed link indicates BioLegend citation): Magatti M, et al. 2008. Stem Cells 26:182. (FC) PubMed
Product Citations: Hutt M, et al. 2018. Oncotarget. 9:11322. PubMed Wallis J, et al. 2020. Eur J Pharm Sci. 152:105456. PubMed Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed Tsoi LC, et al. 2020. JCI Insight. 5:00. PubMed Ishikawa R, et al. 2022. FEBS Open Bio. 12:1178. PubMed Peláez R, et al. 2017. PLoS One.. 10.1371/journal.pone.0181579. PubMed Bennett FC, et al. 2018. Neuron. 98:1170. PubMed Minnella AM, et al. 2018. Sci Rep. 8:17522. PubMed Lu F, et al. 2018. Exp Neurol. 299:65. PubMed Bonavita E, et al. 2020. Immunity. 1215:53. PubMed Yen YT, et al. 2021. Nat Commun. 12:7297. PubMed
RRID: AB_2744505 (BioLegend Cat. No. 401201) AB_2744505 (BioLegend Cat. No. 401202)
Gene ID: NA
Clone: MG2b-57
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ


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