Biolegend, 401207, PE Mouse IgG2b, κ Isotype Ctrl Antibody, 25tests

The isotype of MG2b-57 immunoglobulin is mouse IgG2b,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25tests
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The immunoglobulin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG2b, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular flow cytometry, Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, functional assay. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 401215-401220) as negative control.
Application References(PubMed link indicates BioLegend citation): Magatti M, et al. 2008. Stem Cells 26:182. (FC) PubMed
Product Citations: Li R, et al. 2009. Cell Mol Immunol. 215:6. PubMed Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed Arakawa A, et al. 2021. J Immunol. 207:2235. PubMed
RRID: AB_326637 (BioLegend Cat. No. 401207) AB_326637 (BioLegend Cat. No. 401208)
Gene ID: NA
Clone: MG2b-57
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.