Biolegend, 401322, Alexa Fluor® 647 Mouse IgG3, κ Isotype Ctrl Antibody, 100μg

The isotype of MG3-35 immunoglobulin is mouse IgG3,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: The MG3-35 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401310) as negative control.
Product Citations: Mitchell MW, et al. 2022. Stem Cell Res. 63:102833. PubMed Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed D öpper H, et al. 2020. Stem Cell Research. 72.55486111. PubMed Neureiter A et al. 2018. Stem Cell Res. 33:20-24 . PubMed Schipper L et al. 2018. Stem Cell Res. 33:41-45 . PubMed Bi X, et al. 2017. EBioMedicine. 10.1016/j.ebiom.2017.03.018. PubMed Steenpass L 2017. Stem Cell Res.. 10.1016/j.scr.2017.07.005. PubMed Yamamoto T, et al. 2020. Stem Cell Research. 39:101485.. PubMed Menges J, et al. 2020. Stem Cell Research. 39:101517.. PubMed
RRID: AB_10683445 (BioLegend Cat. No. 401321) AB_10683446 (BioLegend Cat. No. 401322)
Gene ID: NA
Clone: MG3-35
Regulatory Status: RUO
Isotype: Mouse IgG3, κ