Biolegend, 401618, Alexa Fluor® 647 Mouse IgM, κ Isotype Ctrl Antibody, 100μg

The isotype of MM-30 immunoglobulin is mouse IgM, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, and functional assay. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401604) as negative control.
Application References(PubMed link indicates BioLegend citation): Ameres S, et al. 2014. J Immunol. 192:5894. PubMed
Product Citations: Hwang Y, et al. 2014. Sci Rep. 4:5916. PubMed Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed Nur Patria Y, et al. 2020. Stem Cell Research. 42:101689.. PubMed Nur Patria Y, et al. 2020. Stem Cell Research. 72.79305556. PubMed Kim KP, et al. 2021. Cell Stem Cell. 28(7):1291-1306.e10. PubMed Gillespie S, et al. 2018. Br J Pharmacol. 175:3333. PubMed Youngblood B, et al. 2014. J Biotechnol. 172:67. PubMed
RRID: AB_2802167 (BioLegend Cat. No. 401618)
Gene ID: NA
Clone: MM-30
Regulatory Status: RUO
Isotype: Mouse IgM, κ