Biolegend, 402001, Purified Syrian Hamster IgG Isotype Ctrl Antibody, 50μg

The SHG-1 immunoglobulin is of Syrian hamster IgG isotype and was chosen as an isotype control after screening on variety of resting, activated, live, and fixed mouse, rat and human tissues.
50μg
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: This antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICFC, WB, IP, ICC, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: The SHG-1 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays as negative control.
Application References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood. 106:4241.
Product Citations: Huynh NC, et al. 2017. Int J Oral Sci. e5:9. PubMed Watanabe N, et al. 2016. PLoS One. 11: 0150244. PubMed
RRID: AB_326654 (BioLegend Cat. No. 402002)
Gene ID: NA
Clone: SHG-1
Regulatory Status: RUO
Isotype: Syrian Hamster IgG