Product Description
The SHG-1 immunoglobulin is of Syrian hamster IgG isotype and was chosen as an isotype control after screening on variety of resting, activated, live, and fixed mouse, rat and human tissues.
200μg
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The SHG-1 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays as negative control.
Application References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood. 106:4241.
Product Citations: He Z, et al. 2020. J Exp Clin Cancer Res. 39:140. PubMed
RRID: AB_326658 (BioLegend Cat. No. 402006)
Gene ID: NA
Clone: SHG-1
Regulatory Status: RUO
Isotype: Syrian Hamster IgG
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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