Biolegend, 405103, HRP Avidin, 1ml

Avidin binds to biotin with high affinity. Avidin-HRP is useful for detecting biotinylated antibodies, e.g. , in ELISA or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For Western blotting or ELISPOT applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.
1ml
Formulation: Buffered solution containing bovine protein and preservatives (methylisothiazolone, bromonitrodioxane, and other active isothiazolones).
Preparation: Avidin is conjugated with horseradish peroxidase under optimal conditions.
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
Application: ELISA - Quality tested ELISPOT, IHC, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:5000 to determine optimal conditions. For ELISPOT, dilute at 1:500 - 1:2500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Avidin-HRP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Av-HRP is recommended for ELISA, ELISPOT and Western blotting, when used with the relevant substrate system.
Application References(PubMed link indicates BioLegend citation): Qiu LQ, et al. 2012. J Immunol. 188:5150. PubMed McLaughlin HP, et al. 2013. J Med Microbiol. 62:185. PubMed Chan WL, et al. 2013. Nucleic Acids Res. 41:3734. PubMed Macauley MS, et al. 2014. J Biol Chem. 289:351489. PubMed Roncolato EC, et al. 2015. Infect Immun. 83:713. PubMed
Product Citations: Matsuzawa M, et al. 2023. Nat Commun. 14:1417. PubMed Bracho-Sanchez E, et al. 2023. Nat Biomed Eng. . PubMed Soe PP, et al. 2023. Int J Mol Sci. 24:. PubMed Altemus J, et al. 2022. J Cell Physiol. 237:3001. PubMed Vazquez-Lombardi R, et al. 2022. Immunity. 55:1953. PubMed Clausen TM, et al. 2020. Cell. 183:1043. PubMed Montégut L, et al. 2022. STAR Protoc. 3:101095. PubMed Clausen TM, et al. 2020. bioRxiv. . PubMed Roncolato E, et al. 2015. Infect Immun . 83:713. PubMed Qiu L, et al. 2012. J Immunol. 188:5150. PubMed Yuan S, et al. 2021. Nature. 593:418. PubMed Wu J, et al. 2020. Immunity. 53:115. PubMed McLaughlin H, et al. 2013. J Med Microbiol. 62:185. PubMed Chan W, et al. 2013. Nucleic Acids Res. 41:3734. PubMed Tada R, et al. 2015. PLoS One. 10: e0139785. PubMed Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed Macauley M, et al. 2014. J Biol Chem. 289:35149. PubMed Narayanasamy A, et al. 2011. J Proteomics. 74:2948. PubMed Kojima H, et al. 2021. Scand J Immunol. 93:e13020. PubMed Heinzel C, et al. 2021. Front Immunol. 12:753435. PubMed Salehi S, et al. 2017. PLoS One. 10.1371/journal.pone.0163614. PubMed Yabe R, et al. 2021. Nat Commun. 0.565277778. PubMed Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed
Gene ID: NA
UniProt: View information about Avidin on UniProt.org
Regulatory Status: RUO
Other Names: Avidin-Peroxidase, Avidin-Horseradish Peroxidase, Av-HRP