Biolegend, 405215, Cyanine3 Streptavidin, 100μg

Streptavidin binds to biotin with high affinity. Streptavidin-Cyanine3 is useful for detecting biotinylated antibodies. The excitation of Cyanine3 by 550 nm laser light induces a light emission maximum of 570 nm.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with Cyanine3 under optimal conditions.
Concentration: 0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The Streptavidin-Cyanine3 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICC, IHC-F, IHC-P
Recommended Usage: It is recommended that the reagent be titrated for optimal performance for each application. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Cyanine3 conjugation.
Application Notes: The average molecular weight of Streptavidin-Cyanine3 is 60 kD and Streptavidin alone is 52 kD.
Application References(PubMed link indicates BioLegend citation): Heidler J, et al. 2013. Dis Model Mech. 6:1378. PubMed Thoma CR, et al. 2013. J Biomol Screen. 18:1330. PubMed Sunshine J, et al. 2014. J. Virol. 88:1354. PubMed Kai D, et al. 2014. Acta Biomater. 10:2727. PubMed Silginer M, et al. 2014. Cell Death Dis. 5:1012. PubMed
Product Citations: Kai D, et al. 2014. Acta Biomater. 10:2727. PubMed Cacialli P, et al. 2022. EMBO J. 41:e108536. PubMed Franjic D, et al. 2022. Neuron. 110:452. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Atreya I, et al. 2016. J Crohns Colitis. 10: 1132 - 1143. PubMed Kelm W 2013. J Biomol Screen. 18:1330. PubMed Heidler J, et al. 2013. Dis Model Mech. 6:1378. PubMed Silginer M, et al. 2014. Cell Death Dis. 5:1012. PubMed Sunshine J, et al. 2014. J Virol. 88:1354. PubMed Fukuyama T, et al. 2015. J Pharmacol Exp Ther. 354: 394 - 405. PubMed Sharma N, et al. 2015. J Immunol. 195: 5227 - 5236. PubMed Okamura Y, et al. 2017. Allergol Int. 10.1016/j.alit.2017.03.002. PubMed
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: SAv-Cyanine3, SAV