Biolegend, 405218, DyLight™ 488 Streptavidin, 100μg

Streptavidin binds to biotin with high affinity. DyLight™ 488-conjugated Streptavidin is useful for detecting biotinylated antibodies. The excitation of DyLight™ 488 by 493 nm laser light induces a fluorescence emission maximum of 518 nm.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: Streptavidin is conjugated with DyLight™ 488 under optimal conditions.
Concentration: 0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICC, IHC-F, IHC-P
Recommended Usage: This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the DyLight™ 488 conjugation. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Streptavidin-DyLight™ 488 is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-DyLight™ 488 is 60 kD and Streptavidin alone is 52 kD.
Application References(PubMed link indicates BioLegend citation): Ramos TN, et al. 2011. J. Immunol. 186:6657. PubMed. Yan ZX, et al. 2012. Am J Physiol Heart Circ Phyciol. 302:215. PubMed Chen WP, et al. 2014. Cardiovasc Res. 105:44. PubMed
Product Citations: Chen W, et al. 2014. Cardiovasc Res. 105:44. PubMed Ghosh D, et al. 2020. J Cell Sci. . PubMed Fischer A, et al. 2015. Gut. 101136/gutjnl-2015-310022. PubMed Ramos T, et al. 2011. J Immunol. 186:6657. PubMed Yan Z, et al. 2012. Am J Physiol Heart Circ Physiol. 302:H215. PubMed Ghosh D, et al. 2020. J Cell Sci. 133:00:00. PubMed Zhang J, et al. 2022. Int J Biol Sci. 18:6068. PubMed Li X, et al. 2021. Eur J Immunol. 51:2452. PubMed
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: SAv-DyLight™ 488