Biolegend, 405245, PE Streptavidin (High Concentration), 500μg

Streptavidin binds to biotin with high affinity. Streptavidin-Phycoerythrin (PE) is useful for detecting biotinylated antibodies. The excitation of PE by 488 nm laser light induces a light emission maximum of 575 nm.
500μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with PE under optimal conditions.
Concentration: 1.0 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The streptavidin-PE solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Streptavidin-Phycoerythrin (PE) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE is 360 kD and Streptavidin alone is 52 kD.
Application References(PubMed link indicates BioLegend citation): Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. Nishimoto KP,et al. 2008. J. Immunol. 181:4010.PubMed Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed Yamakawa N, et al. 2012. Int Immunol. 25:45. PubMed Shibata T, et al. 2012. Int Immunol. 24:613. PubMed Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed Reading JL, 2013. J. immunol. 190:4542. Pubmed. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
Product Citations: Parry H, et al. 2022. Cell Rep Med. 3:100739. PubMed Shilts J, et al. 2023. PLoS Biol. 21:e3001959. PubMed McDonald J, et al. 2016. J Immunol Methods. 433:6-16. PubMed Malone M, et al. 2022. Methods Mol Biol. 2574:183. PubMed Shilts J, et al. 2022. Nature. 608:397. PubMed Shilts J, et al. 2021. Sci Rep. 0.745138889. PubMed Anisul M, et al. 2021. Elife. 10:. PubMed
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: Streptavidin-Phycoerythrin, SAv-PE
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.