Biolegend, 405247, PE/Dazzle™ 594 Streptavidin, 25μg

Streptavidin binds to biotin with high affinity. Streptavidin - PE/Dazzle™ 594 is useful for detecting biotinylated antibodies. The excitation of PE/Dazzle™ 594 by the 488 nm laser light induces a light emission maximum of 610 nm.
25μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The Streptavidin-PE/Dazzle™ 594 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of Streptavidin-PE/Dazzle™ 594 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE/ Dazzle™ 594 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤0.06 µg per million cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Streptavidin-PE/Dazzle™ 594 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Dazzle™ 594 is 540 kD and Streptavidin alone is 52 kD.
Product Citations: Xu H, et al. 2023. Elife. 12:. PubMed Gao F, et al. 2023. Immunity. 56:864. PubMed Kurane T, et al. 2020. Okajimas Folia Anat Jpn. 96:49:00. PubMed Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed Reed J, et al. 2020. Bio Protoc. 10:e3607. PubMed Panea C, et al. 2021. Commun Biol. 4:913. PubMed Delacher M, et al. 2020. Immunity. 52:295. PubMed Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: Streptavidin-Phycoerythrin/Dazzle™ 594, SAv-PE/Dazzle™ 594