Biolegend, 405281, Spark YG™ 570 Streptavidin, 25μg

Streptavidin binds to biotin with high affinity. Streptavidin-Spark YG™ 570 is useful for detecting biotinylated antibodies. The excitation of Spark YG™ 570 by 555 nm laser light induces a light emission maximum of 570 nm.
25μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration: 0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-F - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Streptavidin-Spark YG™ 570 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Spark YG™ 570 is 60 kD and Streptavidin alone is 52 kD.
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: Streptavidin-Allophycocyanin, SAv-APC