Biolegend, 405303, Biotin Goat anti-mouse IgG (minimal x-reactivity) Antibody, 500μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
500μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed Babayeva M, et al. 2022. Front Immunol. 13:983621. PubMed Cárdenas–Torres FI, et al. 2019. Medicina (Kaunas). 55:3. PubMed Nordgren T, et al. 2015. Transl Res. 166: 57-69. PubMed Brencicova E et al. 2017. Journal of immunological methods. 443:18-25 . PubMed Boulianne B, et al. 2013. J Immunol. 191:4521. PubMed Shen B, et al. 2015. PLoS One. 10: e0141485. PubMed Yuan DJ, et al. 2021. Biomaterials. 273:120797. PubMed Sinha D, et al. 2020. Anal Biochem. 113827:608. PubMed
RRID: AB_315006 (BioLegend Cat. No. 405303)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.