Biolegend, 405305, FITC Goat anti-mouse IgG (minimal x-reactivity) Antibody, 500μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
500μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Timpanaro A, et al. 2023. Int J Mol Sci. 24: . PubMed Zhou HB, et al. 2022. Neoplasma. 69:657. PubMed Jagodinsky JC, et al. 2022. J Immunother Cancer. 10:. PubMed Chang J, et al. 2011. J Immunol. 186:6999. PubMed Sungsuwan S, et al. 2017. Methods Enzymol. 597:359. PubMed Durgeau A, et al. 2018. Nat Commun. 9:5097. PubMed Chang H, et al. 2015. J Endod. 41: 1272-1280. PubMed Fernandez-Arias C, et al. 2013. J Immunol. 190:3363. PubMed Haberland K, et al. 2018. Eur J Immunol. 48:822. PubMed Niemeyer BF, et al. 2021. Nano Sel. . PubMed Indrevær R, et al. 2013. J Immunol. 191:3624. PubMed Liu R, et al. 2015. Am J Physiol Heart Circ Physiol. 309: H1303 - H1313. PubMed Leach S, et al. 2019. Int Immunol. N/A. PubMed Yasuma K, et al. 2016. PLoS Pathog. 12: 1005372. PubMed Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed Ouchida R, et al. 2010. J Immunol. 185:294. PubMed Zhang HC, et al. 2018. Int J Mol Med. 41:13. PubMed Ni X, et al. 2018. Med Sci Monit. 24:1205. PubMed Ni X, et al. 2019. Mol Med Rep. 19:3743. PubMed Jang A, et al. 2021. Front Immunol. 12:701066. PubMed
RRID: AB_315008 (BioLegend Cat. No. 405305)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG