Biolegend, 405306, HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody, 500microl

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
500microl
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Aqueous buffered solution containing a carrier protein and preservatives.
Preparation: The polyclonal antibody was purified from goat antiserum by mouse immunoglobulin affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.This product has a shelf-life of 12 months or less. To obtain a lot-specific expiration date, please enter the lot number in our Certificate of Analysis online tool).
Application: ELISA - Quality tested WB - Verified IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:2,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Song E, et al. 2020. bioRxiv. . PubMed Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed Lo NTN, et al. 2022. J Virol. 96:e0066122. PubMed Knudsen ML, et al. 2022. iScience. 25:105473. PubMed Thaweerattanasinp T, et al. 2023. PeerJ. 11:e14918. PubMed Apte S, et al. 2023. Sci Adv. 9:eade1851. PubMed Weidenbacher PA, et al. 2023. Nat Commun. 14:2149. PubMed Kaur K, et al. 2023. Front Immunol. 14:1132807. PubMed Pibuel MA, et al. 2023. Sci Rep. 13:9356. PubMed Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed Rutkauskaite J, et al. 2022. iScience. 25:104515. PubMed Lužnik Marzidovšek Z, et al. 2022. Am J Pathol. 192:270. PubMed Wright A, et al. 2022. Int J Mol Sci. 23: . PubMed Hsu J, et al. 2022. J Biol Chem. 299:102848. PubMed Wang D, et al. 2023. J Exp Clin Cancer Res. 42:26. PubMed Deich C, et al. 2023. J Biol Eng. 17:4. PubMed Israelow B, et al. 2020. bioRxiv. . PubMed Uka R, et al. 2020. Oncogene. 39:4132. PubMed Vrieling M, et al. 2016. Sci Rep. 6:37759. PubMed Frank N, et al. 2011. Cancer Res. 71:1474. PubMed Chen G, et al. 2018. Front Immunol. 9:549. PubMed Hwang S, et al. 2012. Proc Natl Acad Sci U S A. 110:276. PubMed Boyt DT, et al. 2020. J R Soc Interface. 17:20190815. PubMed Lin YR, et al. 2020. Immunity. 53(4):840-851.e6. PubMed de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed Gupta A, et al. 2020. J Biol Chem. 295:2057. PubMed Copp J, et al. 2014. Proc Natl Acad Sci U S A. 111:13481. PubMed Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed Zhang S, et al. 2021. Front Oncol. 585457:11. PubMed Chiang CF, et al. 2019. Sci Rep. 9:12448. PubMed Parks KR, et al. 2019. Cell Rep. 29:3060. PubMed Baba T, et al. 2021. Cell Death Dis. 12:322. PubMed Sharma A, et al. 2021. Traffic. 22:332. PubMed Yaguchi J, et al. 2021. BMC Biology. 19(1):64. PubMed Martinov T, et al. 2019. J Immunol. 203:844. PubMed Nitiss JL, et al. 2021. Curr Protoc. 1:e250. PubMed Collin R, et al. 2014. J Immunol. 193:3503. PubMed Ventura E, et al. 2018. J Cell Sci. 131. PubMed Song E, et al. 2021. J Exp Med. 218:00:00. PubMed Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed Hoffmann D, et al. 2020. Cancer Immunol Res. 8:19. PubMed Omer S et al. 2018. eLife. 7 pii: e36745. PubMed Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed Furlong K, et al. 2020. J Virol. 94:00:00. PubMed Yan L, et al. 2014. Clin Vaccine Immunol. 21:1206. PubMed Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed Pibuel MA, et al. 2021. Cell Death Discov. 7:280. PubMed Lee K, et al. 2020. Biology (Basel). 9:00. PubMed Powell AE, et al. 2020. bioRxiv. . PubMed Mamedov T, et al. 2021. Front Plant Sci. 12:742875. PubMed Fang Z, et al. 2022. Nat Commun. 13:3250. PubMed Song E, et al. 2021. Cell Rep Med. 2:100288. PubMed Seydoux E, et al. 2021. Cell Reports. 35(5):109084. PubMed Powell AE, et al. 2022. Front Immunol. 13:942897. PubMed Wang K, et al. 2021. EMBO Rep. 22:e52205. PubMed Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed Ohtsuka J, et al. 2021. iScience. Online ahead of print. PubMed Harbour JC, et al. 2021. J Immunol. 206:2596. PubMed Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed Hsing C, et al. 2008. Cytokine. 44:221. PubMed Sertorio M, et al. 2016. J Immunol. 196: 2986-2994. PubMed Hsu HW, et al. 2020. Development. 147:00:00. PubMed Caglar Beker M, et al. 2020. Turk J Med Sci. Online ahead of print. PubMed Ram AK, et al. 2020. World J Gastroenterol. 26:7131. PubMed Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed Khan AA, et al. 2022. Bioconjug Chem. . PubMed Shkoporov A, et al. 2015. FEMS Microbiol Lett. 362:83. PubMed Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed Chung KP, et al. 2019. Nat Commun. 10:3390. PubMed Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed Zhang Q, et al. 2021. Front Cell Infect Microbiol. 11:766922. PubMed Lee SH, et al. 2022. Nat Commun. 13:5461. PubMed Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed Kiianitsa K, et al. 2020. DNA Repair (Amst). 96:102977. PubMed Viereckl MJ, et al. 2022. Biomolecules. 12:. PubMed Klaessens S, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Horton N, et al. 2013. PLoS One. 8:e59552. PubMed Prakash P, et al. 2019. Methods Protoc. 2:48. PubMed Wu YH, et al. 2020. Mol Med Rep. 21:851. PubMed Powell AE, et al. 2021. ACS Cent Sci. 7:183. PubMed Tao L, et al. 2021. Light Sci Appl. 10:179. PubMed Nanna AR, et al. 2020. Nucleic Acids Res. 48:5281. PubMed Ortega-Prieto AM, et al. 2018. Nat Commun. 9:682. PubMed Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed Lun Y, et al. 2021. J Immunol. 206:2819. PubMed Rodems TS, et al. 2022. Commun Biol. 5:897. PubMed Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed Hoffmann D, et al. 2020. Int J Tryptophan Res. 12:1178646919891736. PubMed Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed Miyamoto M, et al. 2018. Oncol Lett. 15:4005. PubMed Han G, et al. 2022. Ann Transl Med. 10:1134. PubMed Harbuzariu A, et al. 2022. iScience. 25:104407. PubMed Huang WC, et al. 2022. Sci Rep. 12:3612. PubMed
RRID: AB_315009 (BioLegend Cat. No. 405306)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG