Biolegend, 405309, Cyanine3 Goat anti-mouse IgG (minimal x-reactivity) Antibody, 100μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
100μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with Cyanine3 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this fluorochrome conjugated antibody is quality control tested by immunofluorescence microscopy. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Lu J, et al. 2023. Mol Cell Biochem. . PubMed Kumagai T, et al. 2022. Cell Biol Int. 46:1530. PubMed McNicoll F, et al. 2020. Life Sci Alliance. 3:e201900564. PubMed Biswas U et al. 2018. Current biology : CB. 28(2):249-261 . PubMed Moriyama S, et al. 2014. EMBO J. 33:1256. PubMed Williams A, et al. 2012. Blood. 120:3069. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Biswas U, et al. 2016. PLoS Genet. 12:e1006389. PubMed Sun ZC, et al. 2021. Front Immunol. 12:762162. PubMed
RRID: AB_893530 (BioLegend Cat. No. 405309)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG