Biolegend, 405315, PE/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Antibody, 100μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
100μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: DeBuque RJ, et al. 2022. Dev Dyn. 251:988. PubMed Zhu B, et al. 2023. Nat Methods. 20:304. PubMed Anderson DJ, et al. 2018. Nat Commun. 9:1373. PubMed Muntasell A, et al. 2016. J Immunol. 196: 3818 - 3827. PubMed Tan J, et al. 2018. Nat Med. 24:401. PubMed Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed Talker S, et al. 2016. J Virol. 90: 9364 - 9382. PubMed Bruveris FF, et al. 2020. Development. 147:00:00. PubMed Li Y, et al. 2021. Front Cell Dev Biol. 9:646077. PubMed Zhou P, et al. 2016. Sci Rep. 6:38597. PubMed Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed López-Montañés M, et al. 2017. J Immunol. 10.4049/jimmunol.1601574. PubMed
RRID: AB_10662421 (BioLegend Cat. No. 405315)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG