Biolegend, 405321, Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) Antibody, 25μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
25μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Chen BR, et al. 2022. Bio Protoc. 12:e4413. PubMed Della Marina A, et al. 2021. Cells. 10:. PubMed Wong JJ, et al. 2021. Sci Adv. 7:. PubMed özdilek A, et al. 2020. Proc Natl Acad Sci U S A. 117:1280. PubMed Jung WH, et al. 2019. Cells. 8:813. PubMed Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed Middleton DR, et al. 2021. J Bacteriol. 203:. PubMed Chen BR, et al. 2021. Elife. 10:. PubMed Pruett L, et al. 2020. Laryngoscope. 130:2432. PubMed Bose S, et al. 2020. Nat Biomed Eng. 4:814. PubMed Khan AA, et al. 2022. Bioconjug Chem. . PubMed Zheng W, et al. 2020. Nature. 577:543. PubMed Smith R, et al. 2017. Biophys J. 10.1016/j.bpj.2017.05.005. PubMed Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed Pandey N, et al. 2020. J Cell Biol. 219:00:00. PubMed Scott WA, et al. 2021. PLoS Genet. 17:e1009909. PubMed
RRID: AB_2563045 (BioLegend Cat. No. 405322)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG