Biolegend, 405324, Alexa Fluor® 555 Goat anti-mouse IgG (minimal x-reactivity) Antibody, 100μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
100μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Deisher A, et al. 2022. STAR Protoc. 3:101663. PubMed Feher A, et al. 2022. Sci Rep. 12:22023. PubMed Fuchs R, et al. 2015. Toxicology. 338: 17-29. PubMed Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed Della Marina A, et al. 2021. Cells. 10:. PubMed Webber M, et al. 2016. Proc Natl Acad Sci U S A. 113(50):14189-14194. PubMed Divito SJ, et al. 2020. J Clin Invest. 130:4624. PubMed Meyers TA, et al. 2020. Hum Mol Genet. 944:29. PubMed Zhu L, et al. 2021. Pharmaceutics. 14:. PubMed Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed Scur M, et al. 2022. Nat Commun. 13:7272. PubMed Iannitti R, et al. 2016. Nat Commun. 7:10791. PubMed van de Veerdonk FL, et al. 2021. J Clin Invest. Online ahead of print. PubMed
RRID: AB_2563179 (BioLegend Cat. No. 405324)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG