Biolegend, 405326, Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Antibody, 100μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
100μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested IHC-F, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Ambade AS, et al. 2019. Transl Res. 203:15. PubMed Farrell K, et al. 2022. Cell Rep. 41:111470. PubMed Aljobaily N, et al. 2022. Nutrients. 15: . PubMed Gurusamy M, et al. 2023. Eur J Med Res. 28:71. PubMed Beaver JT, et al. 2020. Hum Vaccin Immunother. 2.119444444. PubMed Tada T, et al. 2022. Cell Rep. 38:110237. PubMed Beaver JT, et al. 2020. Hum Vaccin Immunother. 2.105555556. PubMed Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed Gao X et al. 2018. Stem cell research. 31:193-196 . PubMed Tada T, et al. 2020. Cell Rep. 33:108528. PubMed Wagner W, et al. 2019. Cell Rep. 28:11. PubMed Antonyshyn JA, et al. 2021. Commun Biol. 4:1205. PubMed
RRID: AB_2563308 (BioLegend Cat. No. 405326)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG