Biolegend, 405356, Spark Blue™ 574 Streptavidin, 100μg

Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii . It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: Streptavidin is conjugated with Spark Blue™ 574 under optimal conditions
Concentration: 0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The streptavidin- Spark Blue™ 574 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this Streptavidin-Spark Blue™ 574 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark Blue™ 574 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL staining volume. It is recommended that the reagent be titrated for optimal performance for each application. * Spark Blue™ 574 has a maximum excitation of 506 nm and a maximum emission of 574 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Streptavidin-Spark Blue™ 574 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Spark Blue™ 574 is 60 kD and Streptavidin alone is 52 kD.
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO