Biolegend, 405401, Purified Goat anti-rat IgG (minimal x-reactivity) Antibody, 500μg

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
500μg
Verified Reactivity: Rat
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA - Quality tested FC, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture, the suggested use of this reagent is ≤ 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
Application References(PubMed link indicates BioLegend citation): Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations: Wang L, et al. 2022. J Gen Physiol. 154:. PubMed Liu PS, et al. 2023. Nat Immunol. 24:452. PubMed Karulf M, et al. 2010. J Immunol. 185:4856. PubMed
RRID: AB_315012 (BioLegend Cat. No. 405401)
Gene ID: 295279
UniProt: View information about IgG on UniProt.org
Clone: Poly4054
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG