Biolegend, 405416, Alexa Fluor® 647 Goat anti-rat IgG (minimal x-reactivity) Antibody, 100μg

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
100μg
Verified Reactivity: Rat
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
Application References(PubMed link indicates BioLegend citation): Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations: Yu L, et al. 2022. Cancer Sci. 113:3980. PubMed Zhang Z, et al. 2022. Cancer Gene Ther. 29:722. PubMed Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed Ghanei Z, et al. 2020. Journal of Cellular Physiology. 235:7840. PubMed Kim H, et al. 2020. ACS Nano. 14:6519. PubMed Duan H, et al. 2020. JCI Insight. 5:00. PubMed Zhang P, et al. 2020. Nat Commun. 11:2487. PubMed Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed Hu J, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.72. PubMed
RRID: AB_2562967 (BioLegend Cat. No. 405416)
Gene ID: 295279
UniProt: View information about IgG on UniProt.org
Clone: Poly4054
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG