Biolegend, 405704, FITC anti-mouse IgD Antibody, 500μg

Surface IgD is an important B cell differentiation marker.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.
Application References(PubMed link indicates BioLegend citation): Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed Xu Y, et al. 2022. Nat Commun. 13:980. PubMed Sweet R, et al. 2011. Proc Natl Acad Sci U S A. 108:7932. PubMed Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed Inoue D, et al. 2021. Nat Genet. 53:707. PubMed Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Mintz MA, et al. 2019. Immunity. 51:310. PubMed Liu X, et al. 2022. Nat Cancer. :. PubMed Zheng S, et al. 2019. PLoS Biol. 17:e3000569. PubMed Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed Yasuda S, et al. 2018. Genes Cells. 23:868. PubMed Ryg-Cornejo V, et al. 2016. Cell Rep. 14:68-81. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID: AB_315025 (BioLegend Cat. No. 405703) AB_315026 (BioLegend Cat. No. 405704)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation
Cell Type: B cells
Biology Area: Immunology
Gene ID: 380797
UniProt: View information about IgD on UniProt.org
Clone: 11-26c.2a
Regulatory Status: RUO
Other Names: Immunoglobulin D
Isotype: Rat IgG2a, κ