Biolegend, 405706, PE anti-mouse IgD Antibody, 200μg

Surface IgD is an important B cell differentiation marker.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.
Application References(PubMed link indicates BioLegend citation): Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Xu Y, et al. 2022. Nat Commun. 13:980. PubMed Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed Santiago M, et al. 2011. J Virol. 85:189. PubMed Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Fistonich C, et al. 2018. J Exp Med. 215:2586. PubMed Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed Chou W, et al. 2006. Blood. 108:3005. PubMed Chen M, et al. 2016. J Immunol . 194: 2607-15. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Cao D, et al. 2011. J Immunol. 187:4451. PubMed Carey J, et al. 2008. J Exp Med. 205:2043. PubMed Nadrah K, et al. 2015. PLoS One. 10: 0136061. PubMed Han S, et al. 2016. Arthritis Res Ther. 0.975. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Duncan CG, et al. 2018. G3 (Bethesda). 0.892361111. PubMed Mosaheb MM, et al. 2017. Front Immunol. 8:225. PubMed Chen C, et al. 2020. Cell Rep. 2136:30. PubMed Xiong Y, et al. 2019. BMC Immunol. 20:24. PubMed Qiu Z, et al. 2020. Cancer Genet. 245:6. PubMed Dörsam B, et al. 2018. Front Immunol. 7:e34961. PubMed Kolan S, et al. 2015. PLoS One. 10: 0134113. PubMed
RRID: AB_315027 (BioLegend Cat. No. 405705) AB_315028 (BioLegend Cat. No. 405706)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation
Cell Type: B cells
Biology Area: Immunology
Gene ID: 380797
UniProt: View information about IgD on UniProt.org
Clone: 11-26c.2a
Regulatory Status: RUO
Other Names: Immunoglobulin D
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.