Biolegend, 405714, APC anti-mouse IgD Antibody, 100μg

Surface IgD is an important B cell differentiation marker.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.
Application References(PubMed link indicates BioLegend citation): Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Pioli KT, et al. 2023. iScience. 26:106223. PubMed Thomsen I, et al. 2021. J Immunol. Online ahead of print. PubMed Xu Y, et al. 2022. Nat Commun. 13:980. PubMed Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed Tian M, et al. 2020. Proc Natl Acad Sci U S A. 117:7929. PubMed Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed Chen S, et al. 2022. Cancer Discov. :. PubMed JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed Luck H, et al. 2019. Nat Commun. 10:3650. PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed Kitabatake M, et al. 2012. J Immunol. 189:1193. PubMed Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
RRID: AB_10645480 (BioLegend Cat. No. 405713) AB_10643423 (BioLegend Cat. No. 405714)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation
Cell Type: B cells
Biology Area: Immunology
Gene ID: 380797
UniProt: View information about IgD on UniProt.org
Clone: 11-26c.2a
Regulatory Status: RUO
Other Names: Immunoglobulin D
Isotype: Rat IgG2a, κ