Biolegend, 406202, Purified anti-mouse IgM, 500μg

This monoclonal antibody was raised by immunization with C57BL/10 mouse splenocytes. It specifically reacts with IgM of Igh-C b and the related haplotypes (d and n), but not with the free μ heavy chain of IgM. Some cross-reaction with IgM of Igh-C e can be detected with this antibody.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, depletion of lymphocytes.
Application References(PubMed link indicates BioLegend citation): Stall AM, et al. 1984. J. Immunol. 132:787. Velardi A, et al. 1984. J. Immunol. 133:2098.
Product Citations: Qiu Q, et al. 2015. J Virol. 3412:89. PubMed
RRID: AB_315035 (BioLegend Cat. No. 406202)
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgMb on UniProt.org
Clone: AF6-78
Regulatory Status: RUO
Other Names: Igh-6b
Isotype: Mouse (BALB/c) IgG1, κ