Biolegend, 406207, PE anti-mouse IgM, 50μg

This monoclonal antibody was raised by immunization with C57BL/10 mouse splenocytes. It specifically reacts with IgM of Igh-C b and the related haplotypes (d and n), but not with the free μ heavy chain of IgM. Some cross-reaction with IgM of Igh-C e can be detected with this antibody.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, depletion of lymphocytes.
Application References(PubMed link indicates BioLegend citation): Stall AM, et al. 1984. J. Immunol. 132:787. Velardi A, et al. 1984. J. Immunol. 133:2098.
Product Citations: Sundling C, et al. 2021. Immunity. 54(5):988-1001.e5. PubMed Noviski M, et al. 2018. Elife. 7:e35074. PubMed Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
RRID: AB_315041 (BioLegend Cat. No. 406208)
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgMb on UniProt.org
Clone: AF6-78
Regulatory Status: RUO
Other Names: Igh-6b
Isotype: Mouse (BALB/c) IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.