Product Description
This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
100μg
Verified Reactivity: Rabbit
Antibody Type: Polyclonal
Host Species: Donkey
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.
Product Citations: Kim D, et al. 2021. Anim Cells Syst (Seoul). 25:264. PubMed Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed Hermanns N, et al. 2022. Basic Res Cardiol. 117:52. PubMed Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed Flommersfeld S, et al. 2021. Immunity. :. PubMed Wu W, et al. 2018. J Am Soc Nephrol. 29:168. PubMed Reinhardt R, et al. 2019. Development. 146:10. PubMed Jin LQ, et al. 2022. Cells. 11:. PubMed Kasagi Y, et al. 2016. Cancer Res . 76: 347 - 357. PubMed
RRID: AB_2563306 (BioLegend Cat. No. 406418)
Gene ID: 100343867
UniProt: View information about Rabbit IgG on UniProt.org
Clone: Poly4064
Regulatory Status: RUO
Isotype: Donkey Polyclonal Ig
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Collaboration
Tony Tang
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