Biolegend, 406421, PE Donkey anti-rabbit IgG (minimal x-reactivity) Antibody, 100μg

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
100μg
Verified Reactivity: Rabbit
Antibody Type: Polyclonal
Host Species: Donkey
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.
Product Citations: Ramovs V, et al. 2019. Breast Cancer Res. 21:63. PubMed Suzuki K, et al. 2019. Int J Mol Med. 44:1187. PubMed Zhang H, et al. 2022. Adv Sci (Weinh). :e2204463. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Wang Y, et al. 2023. iScience. 26:106630. PubMed Eldi P, et al. 2022. Immunol Cell Biol. 100:250. PubMed Zuidema A, et al. 2022. J Cell Biol. 221: . PubMed Asashima H, et al. 2022. J Clin Invest. 132: . PubMed Shukla GS, et al. 2020. Clin Transl Oncol. 22:1094. PubMed Kim EH, et al. 2020. Elife. 9:00. PubMed Komegae EN, et al. 2019. Front Immunol. 10:1496. PubMed Ramovs V, et al. 2020. J Invest Dermatol. . PubMed Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed Hsiue EH, et al. 2021. Science. 371:. PubMed Bencze D, et al. 2022. Int J Mol Sci. 23:. PubMed Liang F, et al. 2021. Front Immunol. 12:658715. PubMed Adler D, et al. 2019. iScience. 15:39. PubMed Zuidema A, et al. 2022. J Cell Sci. 135:. PubMed Alberts A, et al. 2020. Sci Rep. 10:6391. PubMed Min H, et al. 2015. PLoS One. 10: 0138201. PubMed Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed Ryniawec JM, et al. 2022. Diagnostics (Basel). 12:. PubMed Liu Y, et al. 2022. J Inflamm Res. 15:4315. PubMed Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed Shen C, et al. 2021. Front Immunol. 12:680055. PubMed Kim S, et al. 2021. Elife. 10:. PubMed Routhu NK, et al. 2021. Immunity. 54(3):542-556.e9. PubMed Walsh SM, et al. 2021. eLife. 10:00. PubMed Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed Routhu NK, et al. 2021. Nat Commun. 12:3587. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Carmona-Rivera C, et al. 2020. JCI Insight. 5:00. PubMed Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed You C, et al. 2021. Cancers (Basel). 13:. PubMed Secchia S, et al. 2022. Dev Cell. 57:496. PubMed Thirugnanam K, et al. 2019. J Am Heart Assoc. 8:e012792. PubMed Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed Zuidema A, et al. 2018. J Cell Sci. 131. PubMed Kannan S, et al. 2019. Mol Cancer Ther. 1.871527778. PubMed Fujiwara Y, et al. 2020. Clin Transl Immunology. 9:e1162. PubMed Claus M, Wingert S, Watzl C 2016. Open Bio. 6: 160010. PubMed Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed van Nie L, et al. 2020. Sci Rep. 10:11928. PubMed Kwok HS, et al. 2019. ACS Chem Biol. 14:603. PubMed Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
RRID: AB_2563484 (BioLegend Cat. No. 406421)
Gene ID: 100343867
UniProt: View information about Rabbit IgG on UniProt.org
Clone: Poly4064
Regulatory Status: RUO
Isotype: Donkey Polyclonal Ig
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.