Biolegend, 406507, PE anti-mouse IgM Antibody, 50μg

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations: Xu Y, et al. 2022. Nat Commun. 13:980. PubMed Ameres S, et al. 2014. J Immunol. 192:5894. PubMed Forte E, et al. 2020. J Cell Mol Med. . PubMed Jones M, et al. 2016. PLoS One. 11: 0157271. PubMed Suzuki M, et al. 2021. Sci Adv. 7:. PubMed Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed Chen M, et al. 2016. J Immunol . 194: 2607-15. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Webster P, et al. 2018. Nat Commun. 9:2649. PubMed Mansell E, et al. 2020. Cell Stem Cell. . PubMed Aschman T, et al. 2021. Int J Mol Sci. 22:. PubMed Gu X, et al. 2022. Vaccines (Basel). 10:. PubMed Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Lu X, et al. 2019. Cell Rep. 28:472. PubMed Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed Wu J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01677-z. PubMed Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed Roberto MP, et al. 2021. Immunity. 54(8):1807-1824.e14. PubMed Vrijens P, et al. 2019. J Gen Virol. 100:583. PubMed Colacino JA, et al. 2018. Stem Cell Reports. 10:1596. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID: AB_315057 (BioLegend Cat. No. 406507) AB_315058 (BioLegend Cat. No. 406508)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgM on UniProt.org
Clone: RMM-1
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.