Biolegend, 406511, PerCP/Cyanine5.5 anti-mouse IgM Antibody, 25μg

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations: Xu Y, et al. 2022. Nat Commun. 13:980. PubMed Grégoire C, et al. 2022. Immunity. 55:1216. PubMed Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed Fontana MF, et al. 2023. Elife. 12: . PubMed Kim CC et al. 2019. Cell Rep. 27(5):1446-1460 . PubMed Lee JH, et al. 2018. Transgenic Res. 27:241. PubMed Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed Gibb D, et al. 2011. J Immunol. 186:4244. PubMed Nelson SA, et al. 2022. NPJ Vaccines. 7:124. PubMed Patrussi L, et al. 2019. Haematologica. 104:2040. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Werner A, et al. 2021. iScience. 24:103076. PubMed Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID: AB_2075944 (BioLegend Cat. No. 406511) AB_2075943 (BioLegend Cat. No. 406512)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgM on UniProt.org
Clone: RMM-1
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.