Biolegend, 406523, Brilliant Violet 605™ anti-mouse IgM Antibody, 50μg

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations: Caradonna TM, et al. 2022. Cell Rep. 41:111628. PubMed Knudsen ML, et al. 2022. iScience. 25:105473. PubMed Lin YR, et al. 2020. Immunity. 53(4):840-851.e6. PubMed Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed Parks KR, et al. 2019. Cell Rep. 29:3060. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed Lu X, et al. 2019. Cell Rep. 28:472. PubMed Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
RRID: AB_2563358 (BioLegend Cat. No. 406523)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgM on UniProt.org
Clone: RMM-1
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Rat IgG2a, κ