Biolegend, 406604, Biotin anti-mouse IgG1 Antibody, 500μg

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA detection, the suggested use of this reagent is ≤ 1.0 µg per ml. It's recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations: Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed Cao K, et al. 2022. J Virol. 96:e0157821. PubMed MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Babayeva M, et al. 2022. Front Immunol. 13:983621. PubMed Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed Koroleva E, Kobets N 2017. BioMed Res Int. 10.1155/2017/3865802. PubMed He X, et al. 2021. Emerg Microbes Infect. 10:1555. PubMed Collin R, et al. 2014. J Immunol. 193:3503. PubMed Counoupas C, et al. 2020. NPJ Vaccines. 0.28125. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Exposito-Alonso D, et al. 2020. eLife. 9:00. PubMed Dahlgren M, et al. 2015. J Immunol. 194:5187. PubMed
RRID: AB_315062 (BioLegend Cat. No. 406603) AB_315063 (BioLegend Cat. No. 406604)
Gene ID: 16017
UniProt: View information about IgG1 on UniProt.org
Clone: RMG1-1
Regulatory Status: RUO
Other Names: Immunoglobulin G1
Isotype: Rat IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.