Biolegend, 406605, FITC anti-mouse IgG1 Antibody, 50μg

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations: Stoler-Barak L, et al. 2023. Nat Commun. 14:1462. PubMed Safari H, et al. 2022. J Pharm Sci. 111:2525. PubMed Yang K, et al. 2022. Sci Immunol. 7:eabn2888. PubMed Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed Liang Q, et al. 2022. iScience. 25:104043. PubMed Raedler J, et al. 2021. J Clin Immunol. 41:1536. PubMed Geherin S, et al. 2012. J Immunol. 188:6027. PubMed Ma YV, et al. 2021. MAbs. 13:2003281. PubMed Chen M, et al. 2014. PLoS One. 9:107505. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Mintz MA, et al. 2019. Immunity. 51:310. PubMed Le Q, Maizels N 2015. PLoS One. 11: 1005411. PubMed Lam YY, et al. 2020. J Am Heart Assoc. 9:e016528. PubMed Ma X, et al. 2020. Immunity. 53:1315. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Fan Z, et al. 2020. STAR Protoc. 1:100012. PubMed Ghezraoui H, et al. 2018. Nature. 560:122. PubMed Geherin S, et al. 2014. PLoS One. 9:95626. PubMed Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
RRID: AB_493292 (BioLegend Cat. No. 406605) AB_493293 (BioLegend Cat. No. 406606)
Gene ID: 16017
UniProt: View information about IgG1 on UniProt.org
Clone: RMG1-1
Regulatory Status: RUO
Other Names: Immunoglobulin G1
Isotype: Rat IgG