Biolegend, 406610, APC anti-mouse IgG1 Antibody, 200μg

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations: Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed Ivasko SM, et al. 2023. Front Immunol. 13:1023206. PubMed Ehling RA, et al. 2022. Cell Rep. 38:110242. PubMed Bhattacharya Y 2014. J Exp Med. 211:841. PubMed Ishii M, et al. 2021. Pharmaceutics. 13:. PubMed Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed Manresa MC, et al. 2020. Gastroenterology. 159:1778. PubMed Collin R, et al. 2014. J Immunol. 193:3503. PubMed Günal-Sadık G, et al. 2014. PLoS One. 9:111748. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Hare DN, et al. 2021. STAR Protoc. 2:100436. PubMed De Veirman K, et al. 2021. Oncoimmunology. 10:2000699. PubMed Gradiz R, et al. 2016. Sci Rep. 6:21648. PubMed Vergadi E, et al. 2014. J Immunol. 192:394. PubMed Kim S, et al. 2021. Animals (Basel). 11:. PubMed Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed Deen D, et al. 2021. Blood Adv. 5:3002. PubMed Habets T, et al. 2016. PLoS One. 11: 0159515. PubMed Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed Stokes K, et al. 2013. J Virol. 87:10070. PubMed Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed Cronin J, et al. 2016. Mucosal Immunol. 10.1038/mi.2015.131. PubMed Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed Roberts JT, et al. 2020. Mol Cell Proteomics. 19:362. PubMed Jang A, et al. 2021. Front Immunol. 12:701066. PubMed
RRID: AB_10679040 (BioLegend Cat. No. 406609) AB_10696420 (BioLegend Cat. No. 406610)
Gene ID: 16017
UniProt: View information about IgG1 on UniProt.org
Clone: RMG1-1
Regulatory Status: RUO
Other Names: Immunoglobulin G1
Isotype: Rat IgG