Biolegend, 406615, Brilliant Violet 421™ anti-mouse IgG1 Antibody, 125microl

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations: Stoler-Barak L, et al. 2023. Nat Commun. 14:1462. PubMed Lin H, et al. 2015. Mol Ther Methods Clin Dev. 2: 15046. PubMed van den Biggelaar RHGA, et al. 2020. Front Immunol. 11:141. PubMed Biram A, et al. 2020. Cell Rep. 30:1910. PubMed Fumagalli M, et al. 2019. Elife. 8:e44423. PubMed Piao W, et al. 2020. Cell Reports. 30(4):1052-1062.e5.. PubMed Yoshida K, et al. 2020. Mol Cell. 445:78. PubMed Soldevila F, et al. 2018. Front Immunol. 1.625. PubMed
RRID: AB_2562233 (BioLegend Cat. No. 406615) AB_2562234 (BioLegend Cat. No. 406616)
Gene ID: 16017
UniProt: View information about IgG1 on UniProt.org
Clone: RMG1-1
Regulatory Status: RUO
Other Names: Immunoglobulin G1
Isotype: Rat IgG
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.