Biolegend, 406625, Alexa Fluor® 488 anti-mouse IgG1 Antibody, 25μg

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations: Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed Hitachi K, et al. 2023. FASEB J. 37:e22692. PubMed Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed Zavala G, et al. 2020. Front Pharmacol. 0.929861111. PubMed Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
RRID: AB_2715988 (BioLegend Cat. No. 406625) AB_2715989 (BioLegend Cat. No. 406626)
Gene ID: 16017
UniProt: View information about IgG1 on UniProt.org
Clone: RMG1-1
Regulatory Status: RUO
Other Names: Immunoglobulin G1
Isotype: Rat IgG