Biolegend, 406631, Alexa Fluor® 700 anti-mouse IgG1 Antibody, 25μg

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations: Bugaut H, et al. 2024. J Exp Med. :221. PubMed Long CM, et al. 2021. NPJ Vaccines. 6:38. PubMed Tesfamariam M, et al. 2022. Microorganisms. 10:. PubMed
RRID: AB_2783323 (BioLegend Cat. No. 406631) AB_2783324 (BioLegend Cat. No. 406632)
Gene ID: 16017
UniProt: View information about IgG1 on UniProt.org
Clone: RMG1-1
Regulatory Status: RUO
Other Names: Immunoglobulin G1
Isotype: Rat IgG