Biolegend, 407101, Purified anti-mouse IgG2a Antibody, 50μg

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA, FC
Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA capture, the suggested use of this reagent is ≤ 5.0 µg per ml. It's recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Gavin AL, et al.2006. Science 314:1936. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
Product Citations: Wang ZY, et al. 2023. Front Immunol. 1273182:14. PubMed Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed Zelazowska MA, et al. 2020. Life Sci Alliance. :3. PubMed
RRID: AB_345321 (BioLegend Cat. No. 407101) AB_345322 (BioLegend Cat. No. 407102)
Gene ID: 668478
UniProt: View information about IgG2a on UniProt.org
Clone: RMG2a-62
Regulatory Status: RUO
Other Names: Immunoglobulin G2a
Isotype: Rat IgG
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.