Biolegend, 407103, Biotin anti-mouse IgG2a Antibody, 50μg

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA, FC
Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA detection, the suggested use of this reagent is ≤ 1.0 µg per ml. It's recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Gavin AL, et al.2006. Science 314:1936. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
Product Citations: Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed Cao K, et al. 2022. J Virol. 96:e0157821. PubMed Babayeva M, et al. 2022. Front Immunol. 13:983621. PubMed He X, et al. 2021. Emerg Microbes Infect. 10:1555. PubMed Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed Soto JA, et al. 2018. Front Immunol. 9:2875. PubMed Matsuda T, et al. 2022. iScience. 25:105324. PubMed Dahlgren M, et al. 2015. J Immunol. 194:5187. PubMed
RRID: AB_345323 (BioLegend Cat. No. 407103) AB_345324 (BioLegend Cat. No. 407104)
Gene ID: 668478
UniProt: View information about IgG2a on UniProt.org
Clone: RMG2a-62
Regulatory Status: RUO
Other Names: Immunoglobulin G2a
Isotype: Rat IgG
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.