Biolegend, 407108, PE anti-mouse IgG2a Antibody, 100μg

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Gavin AL, et al.2006. Science 314:1936. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
Product Citations: Stabel JR, et al. 2022. PLoS One. 17:e0278313. PubMed Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed Ivasko SM, et al. 2023. Front Immunol. 13:1023206. PubMed Eder JM, et al. 2020. Sci Rep. 10:4045. PubMed An B, et al. 2020. Front Cell Dev Biol. 8:262. PubMed Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed Kim YJ, et al. 2021. Int J Mol Sci. :22. PubMed Lau KX, et al. 2020. Nat Commun. 11:2420. PubMed Habets T, et al. 2016. PLoS One. 11: 0159515. PubMed Song S, et al. 2021. Front Immunol. 12:705140. PubMed Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed
RRID: AB_10549974 (BioLegend Cat. No. 407107) AB_10549456 (BioLegend Cat. No. 407108)
Gene ID: 668478
UniProt: View information about IgG2a on UniProt.org
Clone: RMG2a-62
Regulatory Status: RUO
Other Names: Immunoglobulin G2a
Isotype: Rat IgG
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.