Biolegend, 407303, Biotin anti-mouse Ig light chain λ Antibody, 50μg

The RML-42 monoclonal antibody reacts with immunoglobulin light chain lambda in all tested mouse haplotype (Igh-a and b). It does not react with kappa chain. The RML-42 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA, FC
Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA detection, the suggested use of this reagent is ≤ 1.0 µg per ml. It's recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Ota M, et al. 2010. J. Immunol. 185:4128. PubMed Xu Z, et al. 2012. J. Immunol. 189:4470. PubMed
Product Citations: Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed Abbott RK, et al. 2018. Immunity. 48:133. PubMed
RRID: AB_345331 (BioLegend Cat. No. 407303) AB_345332 (BioLegend Cat. No. 407304)
Biology Area: Immunology
Gene ID: 111519
UniProt: View information about Ig light chain lambda on UniProt.org
Clone: RML-42
Regulatory Status: RUO
Other Names: Imunoglobulin light chain lambda
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.