Biolegend, 407308, PE anti-mouse Ig light chain λ Antibody, 200μg

The RML-42 monoclonal antibody reacts with immunoglobulin light chain lambda in all tested mouse haplotype (Igh-a and b). It does not react with kappa chain. The RML-42 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Ota M, et al. 2010. J. Immunol. 185:4128. PubMed Xu Z, et al. 2012. J. Immunol. 189:4470. PubMed
Product Citations: Pioli KT, et al. 2023. iScience. 26:106223. PubMed Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed Tian M, et al. 2020. Proc Natl Acad Sci U S A. 117:7929. PubMed Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed McGuire A, et al. 2016. Nat Commun. 7:10618. PubMed Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed Noviski M, et al. 2018. Elife. 7:e35074. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed Escolano A, et al. 2016. Cell. 166(6):1445-1458.e12. PubMed
RRID: AB_1027660 (BioLegend Cat. No. 407307) AB_1027659 (BioLegend Cat. No. 407308)
Biology Area: Immunology
Gene ID: 111519
UniProt: View information about Ig light chain lambda on UniProt.org
Clone: RML-42
Regulatory Status: RUO
Other Names: Imunoglobulin light chain lambda
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.